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KMID : 0383119990460020039
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Journal of Aerospace Medicine
1999 Volume.46 No. 2 p.39 ~ p.43
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Urinary Catecholamine Excretion during the G-training
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Yim Sung-Vin
Lee Young-Joon
Lee Moon-Hoon
Choi Woong
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Abstract
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Background : High gravitational acceleration forces are the major stresses which imposed to the pilots and aircrews. They undergo significant psychological and physical changes during flight because of gravitational forces. The human body respond to the various stresses through the neuroendocrine system. Among those, catecholamine system
is the most prompt and powerful defense system to the stress. To evaluate the response of high gravitational forces, urinary catecholamine excretion before and after the flight was analyzed in student pilots of Republic of Korea Air Force Academy using high performance liquid chromatography (HPLC) with fluorescence detection.
Methods : A total of 14 student pilots were exposed to +6GZ for 30 seconds using Human Centrifuge without anti-G suit and urine samples were collected before and after +GZ load. Each urine sample was adjusted to pH 2 with hydrochloric acid and stored at -20℃ and used for HPLC. Urine samples were
derivatized with fluorescence using Toyopak SP cartridge. Concentrations of urinary catecholamine (norepinephrine and epinephrine) were determined using high-performance liquid chromatography (LC-l0A; Shimadzu Co., Kyoto, Japan) with fluorescence detection.
Results : The amount of urinary epinephrine excretion after expose to +6GZ for 30 seconds was significantly increased from 2.49±0.36 to 6.33± 1.81㎍/100㎎ creatinine (154%). Urinary concentration of norepinephrine excretion was increased to 92% (2.22±0.39 to 4.26㎍/100㎎ creatinine). Among 14 trainee, 10 in
epinephrine and 9 in norepinephrine were showed increased or mary excreta ion of each
catecholamine.
Conclusions : In conclusion, high +GZ stress result in increased catecholamine excretion in student pilots. Cartridge derivatization method was very useful method in catecholamine detection. These data suggest that high +GZ stress is a stress stimuli to pilot and secretion patterns of catecholamine can inform us very useful information.
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KEYWORD
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G-training, catecholamine, stress
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